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 Enterobacter cloacae  * and other Enterobacteriaceae strains used for host range screening

Journal: Archives of Virology

Article Title: Three novel Enterobacter cloacae bacteriophages for therapeutic use from Ghanaian natural waters

doi: 10.1007/s00705-024-06081-9

Figure Lengend Snippet: Enterobacter cloacae * and other Enterobacteriaceae strains used for host range screening

Article Snippet: Enterobacter cloacae #5708 , HUSLAB , ++ , - , ++.

Techniques:

Phylogenetic trees of the new Enterobacter phages and their closest relatives. These trees represent the phylogenetic relationships of ( A ) the whole-genome nucleotide sequences and ( B ) the amino acid sequences of the host recognition proteins of the Enterobacter phages fGh-Ecl01, fGh-Ecl02, and fGh-Ecl04 (indicated by red rectangles) to their closest relatives (determined using BLASTn), Escherichia phage T4, and Enterobacteria phage T7. The analysis was done using VICTOR , and the trees were visualized using iTOL . Both trees were rooted at the midpoint. The numbers above the branches represent the branch lengths, and those below the branches are the GBDP pseudo-bootstrap support values from 100 replications. Color-coding: light green, Agtrevirus ; dark green, Aglimvirinae ; light red, Karamvirus ; dark red, Tequatrovirus ; violet, Teseptimavirus

Journal: Archives of Virology

Article Title: Three novel Enterobacter cloacae bacteriophages for therapeutic use from Ghanaian natural waters

doi: 10.1007/s00705-024-06081-9

Figure Lengend Snippet: Phylogenetic trees of the new Enterobacter phages and their closest relatives. These trees represent the phylogenetic relationships of ( A ) the whole-genome nucleotide sequences and ( B ) the amino acid sequences of the host recognition proteins of the Enterobacter phages fGh-Ecl01, fGh-Ecl02, and fGh-Ecl04 (indicated by red rectangles) to their closest relatives (determined using BLASTn), Escherichia phage T4, and Enterobacteria phage T7. The analysis was done using VICTOR , and the trees were visualized using iTOL . Both trees were rooted at the midpoint. The numbers above the branches represent the branch lengths, and those below the branches are the GBDP pseudo-bootstrap support values from 100 replications. Color-coding: light green, Agtrevirus ; dark green, Aglimvirinae ; light red, Karamvirus ; dark red, Tequatrovirus ; violet, Teseptimavirus

Article Snippet: Enterobacter cloacae #5708 , HUSLAB , ++ , - , ++.

Techniques:

Susceptibility of the new Enterobacter cloacae strains to antibiotics and phages. We tested the susceptibility of the two newly isolated E. cloacae strains, #5957 (A-F) and #5958 (G-I), to high (60 µg/ml) and low (1-2 µg/ml) concentrations of meropenem (A, D, and G; low 2 µg/ml), ciprofloxacin (B , E, and H; low 1 µg/ml), and cefepime (C, F, and I; low 1 µg/ml), as well as to the specific phages fGh-Ecl01 (A, B, and C) and fGh-Ecl02 (D, E, and F) for #5957 and fGh-Ecl04 for #5958 (G, H, and I). The optical density (OD 600nm ; y -axis) of the culture was measured every hour for 15 hours (900 minutes; x -axis)

Journal: Archives of Virology

Article Title: Three novel Enterobacter cloacae bacteriophages for therapeutic use from Ghanaian natural waters

doi: 10.1007/s00705-024-06081-9

Figure Lengend Snippet: Susceptibility of the new Enterobacter cloacae strains to antibiotics and phages. We tested the susceptibility of the two newly isolated E. cloacae strains, #5957 (A-F) and #5958 (G-I), to high (60 µg/ml) and low (1-2 µg/ml) concentrations of meropenem (A, D, and G; low 2 µg/ml), ciprofloxacin (B , E, and H; low 1 µg/ml), and cefepime (C, F, and I; low 1 µg/ml), as well as to the specific phages fGh-Ecl01 (A, B, and C) and fGh-Ecl02 (D, E, and F) for #5957 and fGh-Ecl04 for #5958 (G, H, and I). The optical density (OD 600nm ; y -axis) of the culture was measured every hour for 15 hours (900 minutes; x -axis)

Article Snippet: Enterobacter cloacae #5708 , HUSLAB , ++ , - , ++.

Techniques: Isolation